BOUTIQUE精品展示
一 細(xì)胞增殖檢測的意義
細(xì)胞增殖是生物體的重要生命特征,是生物體生長、發(fā)育、繁殖以及遺傳的基礎(chǔ)。監(jiān)測細(xì)胞群的生長對細(xì)胞的狀態(tài)研究很有必要。科研工作者們經(jīng)常需要檢測細(xì)胞增殖情況,比如驗(yàn)證抗腫瘤藥物的藥效時(shí),監(jiān)測細(xì)胞增殖及存活情況;研究腫瘤侵襲與轉(zhuǎn)移模型時(shí),分析細(xì)胞轉(zhuǎn)移或侵襲的路徑;分析創(chuàng)傷的修復(fù)或組織的重建時(shí),檢測細(xì)胞生長情況等等。檢測細(xì)胞增殖及活性變化則可以很好的反映細(xì)胞的生長狀況。
二 細(xì)胞增殖的研究方法
細(xì)胞增殖的研究方法有很多種,根據(jù)檢測原理不同,主要分為DNA合成檢測法、代謝活性檢測法、細(xì)胞增殖標(biāo)志物檢測法等。本文重點(diǎn)介紹DNA合成檢測之Brdu檢測法,傳統(tǒng)DNA合成檢測方法使用胸腺嘧啶核苷(3H-TdR)摻入DNA,然后通過放射自顯影或閃爍計(jì)數(shù)對DNA進(jìn)行定量,該方法由于存在放射性物質(zhì)、實(shí)驗(yàn)周期長、操作繁瑣,且需要昂貴的設(shè)備,目前已被BrdU(5-Bromo-2-deoxyuridine,5-溴脫氧尿嘧啶核苷)檢測方法取代,BrdU作為一種胸苷類似物,可選擇性地?fù)饺朐鲋臣?xì)胞的DNA中,而且可穩(wěn)定存在,并帶到子代細(xì)胞中,細(xì)胞經(jīng)過固定和變性處理后,可用免疫學(xué)方法檢測DNA中BrdU的含量,從而判斷細(xì)胞的增殖能力。
BrdU分子結(jié)構(gòu)
小編整理匯總了常見細(xì)胞增殖檢測方法,詳見下表。
細(xì)胞增殖檢測方法匯總
方法分類 |
檢測方法 |
檢測原理 |
DNA合成檢測 |
Brdu檢測法 |
BrdU是胸腺嘧啶核苷的類似物,能夠在細(xì)胞增殖時(shí)期代替胸苷摻入增殖細(xì)胞的DNA中,而后利用免疫學(xué)方法檢測DNA中BrdU的含量,從而判斷細(xì)胞增殖能力 |
DNA合成檢測 |
胸腺嘧啶核苷(3H-TdR)滲入法 |
用同位素3H標(biāo)記TdR作為DNA合成前體摻入DNA合成代謝過程,通過測定放射性強(qiáng)度,反應(yīng)細(xì)胞DNA代謝及增殖情況 |
代謝活性檢測 |
MTT比色法 |
在活細(xì)胞生長和增殖過程中,線粒體內(nèi)的脫氫酶可將黃色的MTT分解成藍(lán)紫色的Formazan結(jié)晶,再利用酶標(biāo)儀測定,以反映出活細(xì)胞數(shù)目,間接反應(yīng)細(xì)胞增殖情況 |
細(xì)胞增殖標(biāo)志物檢測 |
Ki67,PCNA,MCM-2等相關(guān)抗原檢測 |
通過觀察增殖細(xì)胞中表達(dá)但非增殖細(xì)胞中不存在的特定蛋白質(zhì),來反映細(xì)增殖情況 |
熒光染料檢測 |
羥基熒光素二醋酸鹽琥珀酰亞胺脂(CFSE)檢測法 |
CFSE是一種熒光染料,脂溶性高,進(jìn)入細(xì)胞,可以與胞內(nèi)氨基酸發(fā)生不可逆的結(jié)合,在細(xì)胞的分裂過程中能進(jìn)入子代細(xì)胞,同時(shí)熒光強(qiáng)度減半,再用流式細(xì)胞儀或熒光顯微鏡進(jìn)行分析 |
實(shí)時(shí)動態(tài)電極阻抗檢測 |
RTCA實(shí)時(shí)無標(biāo)記細(xì)胞分析技術(shù) |
將微電極列陣整合在細(xì)胞培養(yǎng)板孔底部,構(gòu)建實(shí)時(shí)、動態(tài)、定量跟蹤細(xì)胞形態(tài)和增殖分化改變的細(xì)胞阻抗檢測傳感系統(tǒng)。當(dāng)微電極表面的細(xì)胞引起貼壁電極界面阻抗的改變時(shí),這種改變與細(xì)胞的實(shí)時(shí)功能狀態(tài)改變呈相關(guān)性 |
三 Exalpha Biologicals品牌BrdU檢測試劑盒
因組織細(xì)胞內(nèi)無內(nèi)源性BrdU存在,可利用BrdU相關(guān)抗體來檢測和定位摻入活躍增殖細(xì)胞新合成DNA的BrdU,Exalpha Biologicals 品牌BrdU檢測試劑盒的優(yōu)勢在于使用細(xì)胞層作為固相,將BrdU試劑摻入微孔板中的細(xì)胞,除了對細(xì)胞增殖情況進(jìn)行評估外,還可以從單個(gè)培養(yǎng)物中分析細(xì)胞數(shù)量、形態(tài)和細(xì)胞抗原等信息。同時(shí)Exalpha Biologicals 品牌BrdU檢測試劑盒可通過試劑盒自帶的固定/變性溶液一步完成細(xì)胞固定、透化和DNA變性等實(shí)驗(yàn)流程,大大縮短了實(shí)驗(yàn)時(shí)間。Exalpha Biologicals 品牌BrdU檢測試劑盒中包含檢測細(xì)胞增殖活性所需的所有組分、操作簡便、無放射性、廣泛的樣本適用性,已得到眾多科研工作者的青睞。
四 Exalpha Biologicals品牌BrdU檢測試劑盒優(yōu)勢
簡便:提供預(yù)稀釋的滴管瓶,現(xiàn)用現(xiàn)配
安全:無放射性成分
完整:包含緩沖液、酶、陽性對照玻片和染色參考玻片
靈活:可用于任何物種組織樣本
靈敏:極低的背景干擾
經(jīng)濟(jì):成本低,高通量,多種規(guī)格
經(jīng)BrdU處理的小鼠小腸免疫組織化學(xué)染色圖
其他西美杰熱賣BrdU相關(guān)產(chǎn)品列表
品牌 |
貨號 |
產(chǎn)品類型 |
產(chǎn)品名稱 |
應(yīng)用 |
Exalpha |
X1327K1 |
試劑試劑盒 |
BrdU Cell Proliferation Assay Kit |
ELISA |
Exalpha |
X1623K1 |
試劑試劑盒 |
BrdU Chemiluminescent Cell Proliferation Assay Kit |
ELISA |
Exalpha |
X1545K |
免疫組化試劑盒 |
BrdU Immunohistochemistry Kit |
ICC |
Cell Biolabs |
CBA-251 |
試劑試劑盒 |
CytoSelect? BrdU Cell Proliferation ELISA Kit |
ELISA |
Exalpha |
X1028 |
BrdU單抗 |
Mouse anti BrdU (bromodeoxyuridine) |
FC、IHC |
Exalpha |
MUB0200P |
BrdU抗體 |
Mouse anti Bromodeoxyuridine / BrdU |
FC、ICC、IHC(frozen & paraffin) |
Exalpha |
A205P |
BrdU多抗 |
Sheep anti BrdU (bromodeoxyuridine) |
FC、IHC(frozen & paraffin)、IP、WB |
Exalpha |
X1543M |
BrdU單抗 |
Mouse anti BrdU (bromodeoxyuridine), conjugated with FITC |
FC、IHC |
Agrisera |
AS16 3995 |
BrdU多抗 |
Bromodeoxyuridine |
IHC、IP、WB |
Agrisera |
AS16 3998 |
BrdU單抗 |
Bromodeoxyuridine (monoclonal, clone MoBU) |
determined by the investigator |
Exalpha |
X2834 |
BrdU試劑 |
Bromodeoxyuridine (BrdU) |
IHC |
西美杰代理的Exalpha Biologicals成立于1998年,是一家位于美國波士頓的生命科學(xué)公司,專門提供尖端抗體、試劑、試劑盒和定制IgY服務(wù)等。2016年5月Exalpha Biologicals與Nordic-MUbio達(dá)成長期銷售協(xié)議,Exalpha Biologicals產(chǎn)品可通過Nordic-MUbio直接銷售給世界各地的客戶。Nordic-MUbio是一家總部位于荷蘭發(fā)展迅猛的抗體試劑公司,Nordic-MUbio提供單克隆抗體、生物標(biāo)志物、即用型流式細(xì)胞術(shù)抗體等產(chǎn)品組合。2022年4月Absolute Biotech將包括Absolute antibody、LSBio、Kerafast、Exalpha、Nordic-MUbio和Everest在內(nèi)多個(gè)生命科學(xué)品牌聯(lián)合成一個(gè)新公司,為世界各地的科研人員提供獨(dú)特抗體、細(xì)胞系、其他試劑及產(chǎn)品定制服務(wù)。欲了解更多產(chǎn)品信息,請?jiān)L問http://www.absolutebiotech.com。
北京西美杰科技有限公司是Absolute Biotech中國區(qū)代理,Exalpha Biologicals代理為用戶提供完善的技術(shù)和售后服務(wù)。如您對上述產(chǎn)品感興趣,歡迎撥打全國客服熱線400-050-4006或登錄官網(wǎng)www.sswptxg.cn查詢和了解。
參考文獻(xiàn)
- Nakamura, S., et al. Application of bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody for the in vivo analysis of proliferative characteristics of human leukemia cells in bone marrows. Oncology 1991, 48, 285-289
- Wilson, G., Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine. Methods Mol. Biol. 1998, 80, 255-266
- Gray, J. (Ed), Special Issue: Monoclonal antibodies against bromodeoxyuridine Cytometry 1985, Vol. 6(6)
- Gutschalk, C.M., et al. ‘Granulocyte Colony-Stimulating Factor and Granulocyte-Macrophage Colony-Stimulating Factor Promote Malignant Growth of Cells from Head and Neck Squamous Cell Carcinomas In vivo.’ Cancer Res., 66, 8026-8036 (2006).
- Gray, J. (Ed), Special Issue: Monoclonal antibodies against bromodeoxyuridine Cytometry 1985, Vol. 6(6)
- Hawker JR Jr., 'Chemiluminescence-based BrdU ELISA to measure DNA synthesis.' J Immunol Methods. 2003 Mar 1;274(1-2):77-82.
- Ang, L.P.K., et al. 'Development of a conjunctival epithelial equivalent with improved proliferative properties using a multistep serum-free culture system.' Investigative Ophthalmology & Visual Science, 2004, 45, 1789-1795
- Dual inhibition of IGF-IR and ALK as an effective strategy to eradicate NPM-ALK+ T-cell lymphoma Bhawana George, Suraj Konnath George, Wenyu Shi, Abedul Haque, Ping Shi, Ghazaleh Eskandari, Magnus Axelson, Olle Larsson, Ahmed O. Kaseb & Hesham M. Amin. Journal of Hematology & Oncologyvolume 12, Article number: 80 (2019). ISSN: 1756-8722
- Wen-Ning Zhao, Norma K. Hylton, Jennifer Wang, Peter S. Chindavong, Begum Alural, Iren Kurtser, Aravind Subramanian, Ralph Mazitschek, Roy H. Perlis, Stephen J. Haggarty. Activation of WNT and CREB Signaling Pathways in Human Neuronal Cells in Response to the Omega-3 Fatty Acid Docosahexaenoic Acid (DHA). Mol Cell Neurosci. 2019 Sep; 99: 103386.
- Eleni Venetsanakos, Ken A. Brameld, Vernon T. Phan, Erik Verner, Timothy D. Owens, Yan Xing, Danny Tam, Jacob LaStant, Kwan Leung, Dane E. Karr, Ronald J. Hill, Mary E. Gerritsen, David M. Goldstein, Jens Oliver Funk, and J. Michael Bradshaw. The Irreversible Covalent Fibroblast Growth Factor Receptor Inhibitor PRN1371 Exhibits Sustained Inhibition of FGFR after Drug Clearance Mol Cancer Ther. 2017, 16(12); 2668–76.
- Nakamura, S., et al. Application of bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody for the in vivo analysis of proliferative characteristics of human leukemia cells in bone marrows. Oncology 1991, 48, 285-289
- Wilson, G., Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine. Methods Mol. Biol. 1998, 80, 255-266
- Gray, J. (Ed), Special Issue: Monoclonal antibodies against bromodeoxyuridine Cytometry 1985, Vol. 6(6)
- Gutschalk, C.M., et al. ‘Granulocyte Colony-Stimulating Factor and Granulocyte-Macrophage Colony-Stimulating Factor Promote Malignant Growth of Cells from Head and Neck Squamous Cell Carcinomas In vivo.’ Cancer Res., 66, 8026-8036 (2006).
- Gray, J. (Ed), Special Issue: Monoclonal antibodies against bromodeoxyuridine Cytometry 1985, Vol. 6(6)